*Thermofisher Scientific 89818

Thermo Scientific Pierce Biotin 3'末端DNA標(biāo)記試劑盒用于標(biāo)記帶有生物素的單鏈DNA引物，用于非放射性電泳遷移率變動(dòng)分析（EMSA）和其他核酸檢測(cè)方法。

DNA生物素化程序使用末端脫氧核苷酸轉(zhuǎn)移酶（TdT）來(lái)催化非模板指導(dǎo)的核苷酸摻入單鏈DNA的3'-OH末端。 TdT表現(xiàn)出對(duì)單鏈DNA的底物偏好，但它將用3'突出端和平端雙鏈體標(biāo)記雙鏈DNA，盡管效率較低。

生物素3'末端DNA標(biāo)記試劑盒的特點(diǎn)：

•非同位素標(biāo)記消除了危險(xiǎn)放射性物質(zhì)或難以處置的廢物的麻煩
•1-3個(gè)生物素化的核糖核苷酸到DNA鏈的3'末端，對(duì)蛋白質(zhì)的雜交或序列特異性結(jié)合的干擾較小
•生物素標(biāo)記的探針?lè)€(wěn)定一年以上
•30分鐘的標(biāo)簽程序快速有效

生物素3'末端DNA標(biāo)記試劑盒已經(jīng)過(guò)優(yōu)化，可將1-3個(gè)生物素化的核糖核苷酸（生物素-11-UTP）整合到DNA鏈的3'末端。該標(biāo)記策略具有將生物素定位于探針的3'末端的優(yōu)點(diǎn)，其中它不太可能干擾蛋白質(zhì)的雜交或序列特異性結(jié)合。生物素標(biāo)記的DNA探針可用于促進(jìn)多種應(yīng)用中的非同位素檢測(cè)，包括電泳遷移率變動(dòng)分析（EMSA），Northern或Southern印跡，菌落雜交或原位雜交。

*Thermofisher Scientific 89818

The Thermo Scientific Pierce Biotin 3' End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use in non-radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.

The DNA biotinylation procedure uses terminal deoxynucleotidyl transferase (TdT) to catalyze nontemplate-directed nucleotide incorporation onto the 3'-OH end of single-stranded DNA. TdT exhibits a substrate preference of single-stranded DNA, but it will label duplex DNA with 3' overhangs and blunt duplexes, albeit with a lower efficiency.

Features of the Biotin 3' End DNA Labeling Kit:

• Non-isotopic labeling eliminates the hassle of hazardous radioactive materials or difficult-to-dispose-of waste
• 1-3 biotinylated ribonucleotides onto the 3' end of DNA strands for less interference with hybridization or sequence-specific binding of proteins
• Biotin-labeled probes are stable for more than one year
• 30-minute labeling procedure is fast and efficient

The Biotin 3' End DNA Labeling Kit has been optimized to incorporate 1-3 biotinylated ribonucleotides (Biotin-11-UTP) onto the 3' end of DNA strands. This labeling strategy has the advantage of localizing the biotin to the 3' end of the probe where it will be less likely to interfere with hybridization or sequence-specific binding of proteins. Biotin-labeled DNA probes can be used to facilitate non-isotopic detection in a variety of applications including electrophoretic mobility shift assays (EMSA), Northern or Southern blots, colony hybridizations or in situ hybridizations.